Non-invasive quantitative detection and applications of non-toxic, S65T-type green fluorescent protein in living plants.

@article{Niwa1999NoninvasiveQD,
  title={Non-invasive quantitative detection and applications of non-toxic, S65T-type green fluorescent protein in living plants.},
  author={Yasuo Niwa and Tomoharu Hirano and Kohki Yoshimoto and Masanori Shimizu and Hiroko Kobayashi},
  journal={The Plant journal : for cell and molecular biology},
  year={1999},
  volume={18 4},
  pages={455-63}
}
Green fluorescent protein (GFP) has emerged as a powerful new tool in a variety of organisms. An engineered sGFP(S65T) sequence containing optimized codons of highly expressed eukaryotic proteins has provided up to 100-fold brighter fluorescence signals than the original jellyfish GFP sequence in plant and mammalian cells. It would be useful to establish a non-invasive, quantitative detection system which is optimized for S65T-type GFP, one of the brightest chromophore mutants among the various… CONTINUE READING
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