Non-injection methods for the production of embryonic stem cell-embryo chimaeras.

Abstract

The simple aggregation of pluripotent ES cells with morulae-stage embryos can result in viable chimaeras at a similar frequency to that of blastocyst injection. The savings in time and equipment inherent in the aggregation techniques, however, make this approach well worth considering.

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@article{Wood1993NoninjectionMF, title={Non-injection methods for the production of embryonic stem cell-embryo chimaeras.}, author={S. A. Wood and Nicholas D. Allen and Janet Rossant and Anna B. Auerbach and Andr{\'a}s Nagy}, journal={Nature}, year={1993}, volume={365 6441}, pages={87-9} }