Non-glycosylphosphatidylinositol (GPI)-anchored recombinant prion protein with dominant-negative mutation inhibits PrPSc replication in vitro.

@article{Kishida2004NonglycosylphosphatidylinositolR,
  title={Non-glycosylphosphatidylinositol (GPI)-anchored recombinant prion protein with dominant-negative mutation inhibits PrPSc replication in vitro.},
  author={Hitaru Kishida and Yuji Sakasegawa and Kota Watanabe and Yoshio Yamakawa and Masahiro Nishijima and Yoshiyuki Kuroiwa and Naomi S Hachiya and Kiyotoshi Kaneko},
  journal={Amyloid : the international journal of experimental and clinical investigation : the official journal of the International Society of Amyloidosis},
  year={2004},
  volume={11 1},
  pages={14-20}
}
Dominant-negative mouse prion protein (PrP) with a lysine mutation at codon 218 (Q218K) is known to inhibit prion replication. In order to gain further mechanistic insight into such dominant negative inhibition, non-glycosylphosphatidylinositol (GPI)-anchored recombinant PrP with Q218K (rPrP-Q218K) was investigated. When applied into scrapie-infected mouse neuroblastoma (ScN2a) cells, rPrP-Q218K but not wild-type rPrP (rPrP-WT) exclusively inhibited abnormal protease-resistant pathogenic… CONTINUE READING

Similar Papers

Loading similar papers…