Non-genetic population heterogeneity studied by in situ polymerase chain reaction.

@article{TolkerNielsen1998NongeneticPH,
  title={Non-genetic population heterogeneity studied by in situ polymerase chain reaction.},
  author={Tim Tolker-Nielsen and Kim Holmstr\om and Lars Boe and Soeren Molin},
  journal={Molecular microbiology},
  year={1998},
  volume={27 6},
  pages={1099-105}
}
Expression of a lac operon in Salmonella typhimurium single cells was monitored using lac mRNA targeting in situ reverse transcription-polymerase chain reaction (RT-PCR). It is demonstrated that suboptimal induction of the lac operon in a culture of S. typhimuriuml/F'lac+ cells generates a subpopulation in which transcription of the lac operon occurs and another subpopulation in which transcription of the lac operon is repressed, whereas suboptimal induction of the lac operon in a culture of S… CONTINUE READING

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