Non-enzymatic transcription of an oligodeoxynucleotide 14 residues long.

  title={Non-enzymatic transcription of an oligodeoxynucleotide 14 residues long.},
  author={Oscar L. Acevedo and Leslie E. Orgel},
  journal={Journal of molecular biology},
  volume={197 2},
  • O. Acevedo, L. Orgel
  • Published 20 September 1987
  • Biology, Chemistry
  • Journal of molecular biology
Pyranosyl‐RNA: Further Observations on Replication
Replication (single-turnover) of pyranosyl-RNA (p-RNA) sequences can be accomplished reliably by template-directed ligation of 2',3'-cyclophosphates of short oligomers. The ligation process was
Preference for Internucleotide Linkages as a Function of the Number of Constituents in a Mixture
The surprising finding was that, under identical conditions, including the same total monomer concentration, the product distribution differs substantially from one reaction to another, most likely due to changing intermolecular interactions depending on the constituents.
The limits of template-directed synthesis with nucleoside-5′-phosphoro(2-methyl)imidazolides
It is shown that isolated A or T residues within an oligo(G) sequence are a complete block to copying and that an isolated C residue is copied inefficiently.
Template-directed extension of a guanosine 5′-phosphate covalently attached to an oligodeoxycytidylate template
The pG residue acts as a primer and is extended very efficiently by incubation with activated pG derivatives to give products containing 6–9 G residues in >80% yield.
Chemical self-replication of palindromic duplex DNA
Self-replication of palindromic duplex DNA-like oligonucleotides, 24 monomers long, in the absence of enzymes is reported by means of a cycle that transfers information from template to copy and is potentially capable of extension to include non-symmetrical sequences, selection and mutation.
Information transfer from peptide nucleic acids to RNA by template-directed syntheses.
It is shown that information can be transferred from PNA to RNA, and the reactions of activated mononucleotides on heterosequences of PNA are described.
The origin of polynucleotide-directed protein synthesis
  • L. Orgel
  • Biology
    Journal of Molecular Evolution
  • 2007
It is suggested that the simple attachment of amino acids to the 2′(3′)-termini of RNA templates favored initiation of replication at the end of the template rather than at internal positions.
Prebiotic chemistry and the origin of the RNA world.
  • L. Orgel
  • Biology
    Critical reviews in biochemistry and molecular biology
  • 2004
The demonstration that ribosomal peptide synthesis is a ribozyme-catalyzed reaction makes it almost certain that there was once an RNA World, and a discussion of genetic systems simpler than RNA that might have "invented" RNA is discussed.
A modified cellular automata model of nucleotide interactions and non-enzymatic transcription of DNA
A simple, flexible and accurate computer model of nucleotide interactions that lead to the non-enzymatic transcription of an oligon nucleotide that acts as a template to catalyze the formation of a suite of oligonucleotides is developed.
The binding and reactions of nucleotides and polynucleotides on iron oxide hydroxide polymorphs
The iron oxide hydroxide minerals goethite and akaganéite were likely constituents of the sediments present in, for instance, geothermal regions of the primitive earth. They may have adsorbed


Template-directed synthesis on the oligonucleotide d(C7-G-C7).
A nonenzymatic RNA polymerase model.
Polynucleotide templates containing C (cytidine) as the major component facilitate the synthesis of oligonucleotides from mixtures of the activated mononucleotide derivatives and produces products with mean chain lengths of six to ten nucleotides.
Mapping adenines, guanines, and pyrimidines in RNA.
The positions of adenines, guanines, and pyrimidines can be determined by partial nuclease digestion of a terminally labeles RNA molecule and form the basis of an RNA sequencing method and are demonstrated on yeast 5.8S ribosomal RNA.