Non-enzymatic hydrolysis of creatine ethyl ester.

  title={Non-enzymatic hydrolysis of creatine ethyl ester.},
  author={Nicholas S Katseres and David W. Reading and Luay Shayya and John C Dicesare and Gordon H Purser},
  journal={Biochemical and biophysical research communications},
  volume={386 2},
The rate of the non-enzymatic hydrolysis of creatine ethyl ester (CEE) was studied at 37 degrees C over the pH range of 1.6-7.0 using (1)H NMR. The ester can be present in solution in three forms: the unprotonated form (CEE), the monoprotonated form (HCEE(+)), and the diprotonated form (H(2)CEE(2+)). The values of pK(a1) and pK(a2) of H(2)CEE(2+) were found to be 2.30 and 5.25, respectively. The rate law is found to be Rate=-dCCEE/dt=k++[H2CEE2+][OH-]+k+[HCEE+][OH-]+k0[CEE][OH-] where the rate… CONTINUE READING