Non-base-contacting residues enable kaleidoscopic evolution of metazoan C2H2 zinc finger DNA binding

@inproceedings{Najafabadi2017NonbasecontactingRE,
  title={Non-base-contacting residues enable kaleidoscopic evolution of metazoan C2H2 zinc finger DNA binding},
  author={Hamed S. Najafabadi and Michael Garton and Matthew T. Weirauch and Sanie Mnaimneh and Ally Yang and Philip M. Kim and Timothy R. Hughes},
  booktitle={Genome Biology},
  year={2017}
}
The C2H2 zinc finger (C2H2-ZF) is the most numerous protein domain in many metazoans, but is not as frequent or diverse in other eukaryotes. The biochemical and evolutionary mechanisms that underlie the diversity of this DNA-binding domain exclusively in metazoans are, however, mostly unknown. Here, we show that the C2H2-ZF expansion in metazoans is facilitated by contribution of non-base-contacting residues to DNA binding energy, allowing base-contacting specificity residues to mutate without… CONTINUE READING