Non-SELEX: selection of aptamers without intermediate amplification of candidate oligonucleotides

@article{Berezovski2006NonSELEXSO,
  title={Non-SELEX: selection of aptamers without intermediate amplification of candidate oligonucleotides},
  author={Maxim V Berezovski and Michael U. Musheev and Andrei P Drabovich and Julia V Jitkova and Sergey N Krylov},
  journal={Nature Protocols},
  year={2006},
  volume={1},
  pages={1359-1369}
}
Aptamers are typically selected from libraries of random DNA (or RNA) sequences through systematic evolution of ligands by exponential enrichment (SELEX), which involves several rounds of alternating steps of partitioning of candidate oligonucleotides and their PCR amplification. Here we describe a protocol for non-SELEX selection of aptamers — a process that involves repetitive steps of partitioning with no amplification between them. Non-equilibrium capillary electrophoresis of equilibrium… CONTINUE READING

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addressing the polymerase chain reaction issue

  • M. U. Musheev, Krylov, S. N. Selection of aptamers by systematic evolution o enrichment
  • Anal. Chim. Acta 564, 91–96
  • 2006

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