Non-CpG methylation is prevalent in embryonic stem cells and may be mediated by DNA methyltransferase 3a.

@article{Ramsahoye2000NonCpGMI,
  title={Non-CpG methylation is prevalent in embryonic stem cells and may be mediated by DNA methyltransferase 3a.},
  author={Bernard H. Ramsahoye and Detlev Biniszkiewicz and Frank Lyko and Virginia Clark and Adrian Bird and Rudolf Jaenisch},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2000},
  volume={97 10},
  pages={5237-42}
}
Current evidence indicates that methylation of cytosine in mammalian DNA is restricted to both strands of the symmetrical sequence CpG, although there have been sporadic reports that sequences other than CpG may also be methylated. We have used a dual-labeling nearest neighbor technique and bisulphite genomic sequencing methods to investigate the nearest neighbors of 5-methylcytosine residues in mammalian DNA. We find that embryonic stem cells, but not somatic tissues, have significant cytosine… CONTINUE READING
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