NompC TRP Channel Required for Vertebrate Sensory Hair Cell Mechanotransduction

  title={NompC TRP Channel Required for Vertebrate Sensory Hair Cell Mechanotransduction},
  author={Samuel Sidi and Rainer W. Friedrich and Teresa Nicolson},
  pages={96 - 99}
The senses of hearing and balance in vertebrates rely on the sensory hair cells (HCs) of the inner ear. The central element of the HC's transduction apparatus is a mechanically gated ion channel of unknown identity. Here we report that the zebrafish ortholog of Drosophila no mechanoreceptor potential C (nompC), which encodes a transient receptor potential (TRP) channel, is critical for HC mechanotransduction. In zebrafish larvae, nompC is selectively expressed in sensory HCs. Morpholino… 
TRPA1 is a candidate for the mechanosensitive transduction channel of vertebrate hair cells
Inhibition of TRPA1 protein expression in zebrafish and mouse inner ears inhibits receptor cell function, as assessed with electrical recording and with accumulation of a channel-permeant fluorescent dye.
Drosophila TRPN( = NOMPC) Channel Localizes to the Distal End of Mechanosensory Cilia
The results provide a strong evidence for NOMPC as a primary transduction channel in Drosophila mechansensory organs and reveals a structural basis for the model of auditory chordotonal transduction in which the TRPN and TRPV channels play sequential roles in generating and amplifying the receptor potential, but have opposing roles in regulating active ciliary motility.
Xenopus TRPN1 (NOMPC) localizes to microtubule-based cilia in epithelial cells, including inner-ear hair cells.
The cloned TRPN1 ortholog from the amphibian Xenopus laevis, generated an antibody against the protein, and determined the protein's cellular and subcellular localization suggest that although TRPN 1 is unlikely to be the transduction channel of stereocilia, it plays an essential role, functionally related to transduction, in the kinocilium.
Are TMCs the Mechanotransduction Channels of Vertebrate Hair Cells?
Two strong candidates, TMC1 and TMC2 (transmembrane channel-like), have emerged from discovery of deafness genes in humans and mice, and the great majority of evidence is consistent with these TMCs as pore-forming subunits of the long-sought hair-cell transduction channel.
Molecular Structure of the Hair Cell Mechanoelectrical Transduction Complex.
Recent findings on the identification and function of proteins that are components of the MET machinery in hair cells are reviewed and remaining open questions are considered.
gemini Encodes a Zebrafish L-Type Calcium Channel That Localizes at Sensory Hair Cell Ribbon Synapses
It is shown that mutations in a zebrafish LTCC encoding gene, cav1.3a, underlie the auditory-vestibular defects of gemini (gem) circler mutants, and that the bulk of Gem/Cav1.
Two Interdependent TRPV Channel Subunits, Inactive and Nanchung, Mediate Hearing in Drosophila
The IAV protein forms a hypotonically activated channel when expressed in cultured cells; in flies, it is specifically expressed in the chordotonal neurons, localized to their cilia and required for hearing.
Ankyrin Repeats Convey Force to Gate the NOMPC Mechanotransduction Channel
Fishing for key players in mechanotransduction
Transient Receptor Potential Melastatin 1: A Hair Cell Transduction Channel Candidate
Hearing thresholds were evaluated in adult mice with auditory-evoked brain stem responses and data suggest that TRPM1 is not essential for development of hearing or balance and it is unlikely that it is a component of the hair cell MET channel.


A Drosophila mechanosensory transduction channel.
This data indicates that the no mechanoreceptor potential C (nompC) gene encodes a new ion channel that is essential for mechanosensory transduction, and D. melanogaster NOMPC and a Caenorhabditis elegans homolog were selectively expressed in mechanos Sensory organs.
Molecular basis of mechanosensory transduction
Progress in both invertebrates and vertebrates is beginning to reveal the identities of proteins essential for transduction, and the scarcity of these cells and the uniqueness of their transduction mechanisms have conspired to slow molecular characterization of the ensembles that carry out mechanotransduction.
Defective calmodulin-dependent rapid apical endocytosis in zebrafish sensory hair cell mutants.
The presence of endocytic defects in the majority of zebrafish mechanosensory mutants points to a important role of apical endocytosis in hair cell function.
The trp ion channel family
Three subgroups comprise the TRP channel family; the best understood of these mediates responses to painful stimuli, and other proposed functions include repletion of intracellular calcium stores, receptor-mediated excitation and modulation of the cell cycle.
The hair cell's transduction channel
OSM-9, A Novel Protein with Structural Similarity to Channels, Is Required for Olfaction, Mechanosensation, and Olfactory Adaptation inCaenorhabditis elegans
Although cyclic nucleotide-gated channels mediate sensory transduction in olfaction and vision, other forms of sensory transduction are independent of these channels. Caenorhabditis elegans cyclic