Noladin ether, a putative novel endocannabinoid: inactivation mechanisms and a sensitive method for its quantification in rat tissues

@article{Fezza2002NoladinEA,
  title={Noladin ether, a putative novel endocannabinoid: inactivation mechanisms and a sensitive method for its quantification in rat tissues},
  author={Filomena Fezza and Tiziana Bisogno and Alberto Minassi and Giovanni Appendino and Raphael Mechoulam and Vincenzo Di Marzo},
  journal={FEBS Letters},
  year={2002},
  volume={513}
}

Ether‐linked analogue of 2‐arachidonoylglycerol (noladin ether) was not detected in the brains of various mammalian species

It is found that 2‐AG ether is not present, at least in an appreciable amount, in the rat brain by gas chromatography‐mass spectrometry analysis and fluorometric high performance liquid chromatography analysis.

Resorcinol-sn-glycerol derivatives: novel 2-arachidonoylglycerol mimetics endowed with high affinity and selectivity for cannabinoid type 1 receptor.

The cannabinoid receptor binding properties, the CB1 functional activity, and the stability to plasma esterases of a novel series of compounds characterized by the conversion of the amide head into the glycerol-ester or glycerl-ether head, typical of 2-AG or the "putative" endocannabinoid 2-AGE, respectively are explored.

The endocannabinoid system: a general view and latest additions

The latest ‘additions’ to the endocannabinoid system are reviewed together with earlier breakthroughs that have contributed to the present knowledge of the biochemistry and pharmacology of theendocannabinoids.

Simultaneous determination of 2-arachidonoylglycerol, 1-arachidonoylglycerol and arachidonic acid in mouse brain tissue using liquid chromatography/tandem mass spectrometry.

A sensitive and specific quantification method is developed for simultaneous determination of 2-AG, 1-AG and AA from mouse brain and adipose tissues by liquid chromatography/tandem mass spectrometry (LC/MS/MS) using a simple brain sample preparation method.

A simple LC-MS/MS method for the simultaneous quantification of endocannabinoids in biological samples.

Comparison of the enzymatic stability and intraocular pressure effects of 2-arachidonylglycerol and noladin ether, a novel putative endocannabinoid.

Noladin ether degraded more slowly than either 2-AG or AEA in the iris-ciliary body and cornea homogenates, probably because of its more stable chemical structure.

Determination of endocannabinoids in nematodes and human brain tissue by liquid chromatography electrospray ionization tandem mass spectrometry.

The Endocannabinoid Noladin Ether Acts as a Full Agonist at Human CB2 Cannabinoid Receptors

Surprisingly, in transfected CHO cells, NE exhibits a relatively high nanomolar affinity for CB2 receptors, comparable to that observed for the endocannabinoid 2-arachidonoyl glycerol (2-AG) (Ki = 1016 nM), which indicates that NE acts as a full agonist at human CB2 receptor and thus might have important physiological functions at peripheral cannabinoid receptors.
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