Noise suppression of point spread functions and its influence on deconvolution of three-dimensional fluorescence microscopy image sets.

@article{Lai2005NoiseSO,
  title={Noise suppression of point spread functions and its influence on deconvolution of three-dimensional fluorescence microscopy image sets.},
  author={Xingyu Lai and Zhiping Lin and E. Sally Ward and R. J. Ober},
  journal={Journal of microscopy},
  year={2005},
  volume={217 Pt 1},
  pages={
          93-108
        }
}
The point spread function (PSF) is of central importance in the image restoration of three-dimensional image sets acquired by an epifluorescent microscope. Even though it is well known that an experimental PSF is typically more accurate than a theoretical one, the noise content of the experimental PSF is often an obstacle to its use in deconvolution algorithms. In this paper we apply a recently introduced noise suppression method to achieve an effective noise reduction in experimental PSFs. We… CONTINUE READING

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