No filters, no fridges: a method for preservation of water samples for eDNA analysis

  title={No filters, no fridges: a method for preservation of water samples for eDNA analysis},
  author={Kelly E Williams and K. Huyvaert and Antoinette J Piaggio},
  journal={BMC Research Notes},
  • Kelly E Williams, K. Huyvaert, Antoinette J Piaggio
  • Published 2016
  • Biology, Medicine
  • BMC Research Notes
  • BackgroundAdvancements in the detection of environmental DNA (eDNA) for detecting species of interest will likely allow for expanded use of these techniques in the field. One obstacle that continues to hinder applications in the field is the requirement of a cold chain of storage for water samples containing eDNA. While eDNA has been successfully preserved using Longmire’s lysis buffer applied to filters, it has yet to be tried with freshwater samples collected for eDNA detection of an invasive… CONTINUE READING
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    Publications referenced by this paper.
    Noncryogenic Preservation of Mammalian Tissues for DNA Extraction: An Assessment of Storage Methods
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    Preservation of RNA and DNA from mammal samples under field conditions
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    • Open Access