No evidence for interference of h&e staining in DNA testing: usefulness of DNA extraction from H&E-stained archival tissue sections.

@article{Morikawa2012NoEF,
  title={No evidence for interference of h&e staining in DNA testing: usefulness of DNA extraction from H&E-stained archival tissue sections.},
  author={Teppei Morikawa and Kaori Shima and Aya Kuchiba and Mai Yamauchi and Noriko Tanaka and Yasunobe Imamura and Xiaoyun Liao and Zhi Rong Qian and Mohan Brahmandam and Janina A. Longtine and Neal I. Lindeman and Charles S. Fuchs and Shuji Ogino},
  journal={American journal of clinical pathology},
  year={2012},
  volume={138 1},
  pages={
          122-9
        }
}
Although histochemical staining has been believed to inhibit the DNA amplification reaction, no previous study has systematically evaluated the influence of histochemical staining on downstream molecular assays. To evaluate an influence of H&E staining on DNA testing, we isolated DNA from 10 unstained, 10 hematoxylin-stained, 10 eosin-stained, and 10 H&E-stained tissue sections (ie, 4 groups), from each of 5 colon cancers. Among the 4 groups, we did not observe any significant or appreciable… CONTINUE READING
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