No complement receptor 1 stumps on podocytes in human glomerulopathies.

Abstract

BACKGROUND Type one complement receptor (CR1) is the only physiological inhibitor of complement on podocytes. CR1 is lost in different glomerulopathies, in particular in lupus nephritis, in which it has been suggested that CR1 is removed by proteolysis from the cell membrane. METHODS To define whether proteolytic cleavage of CR1 on podocytes is a general phenomenon, we analyzed the expression of CR1 in different glomerulopathies using a monoclonal antibody against epitopes present on the extracellular portion of the molecule and a polyclonal antibody directed at the intracellular tail of CR1. The two antibodies were applied on sequential serial histologic sections of renal biopsy. RESULTS In normal glomeruli, the two antibodies provided similar results, that is, strong staining of podocytes, and both were shown to recognize specifically CR1. Decreased expression of the extracellular portion of CR1 was observed in lupus nephritis (8/8), focal and segmental glomerulosclerosis (FSGS; 7/7), IgA nephritis (6/6), membranous glomerulonephritis (3/3), and minimal change disease (3/3). In each case, the decreased expression was accompanied by a simultaneous decrease of the expression of the intracellular tail of CR1 (Spearman's correlation coefficient rs = 0.951, P < 0.001). This observation was confirmed by analyzing focal glomerular lesions on sequential serial sections. CONCLUSION These data indicate that there are no CR1 stumps on podocytes, even in lupus nephritis, and suggest that the CR1 loss on podocytes is not due to consumption but to decreased synthesis. A loss of CR1 synthesis might render podocytes highly sensitive to complement attack.

0100200300'00'02'04'06'08'10'12'14'16
Citations per Year

1,000 Citations

Semantic Scholar estimates that this publication has 1,000 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Moll2001NoCR, title={No complement receptor 1 stumps on podocytes in human glomerulopathies.}, author={Solange Moll and Sylvie Miot and Salima Sadallah and Fred G. Gudat and Michael J{\"{o}rg Mihatsch and J{\"{u}rg Alfred Schifferli}, journal={Kidney international}, year={2001}, volume={59 1}, pages={160-8} }