NmeCas9 is an intrinsically high-fidelity genome-editing platform

@inproceedings{Amrani2018NmeCas9IA,
  title={NmeCas9 is an intrinsically high-fidelity genome-editing platform},
  author={Nadia Amrani and Xin D Gao and Pengpeng Liu and Alireza Edraki and Aamir Mir and Raed Ibraheim and Ankit Gupta and Kanae E. Sasaki and Tong Wu and Paul Daniel Donohoue and Alexander H. Settle and Alexandra M. Lied and Kyle Mcgovern and Chris K. Fuller and Peter Cameron and Thomas G Fazzio and Lihua Julie Zhu and Scot A. Wolfe and Erik J Sontheimer},
  booktitle={Genome Biology},
  year={2018}
}
BackgroundThe development of CRISPR genome editing has transformed biomedical research. Most applications reported thus far rely upon the Cas9 protein from Streptococcus pyogenes SF370 (SpyCas9). With many RNA guides, wildtype SpyCas9 can induce significant levels of unintended mutations at near-cognate sites, necessitating substantial efforts toward the development of strategies to minimize off-target activity. Although the genome-editing potential of thousands of other Cas9 orthologs remains… CONTINUE READING
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