Nm-seq maps 2′-O-methylation sites in human mRNA with base precision

@article{Dai2017NmseqM2,
  title={Nm-seq maps 2′-O-methylation sites in human mRNA with base precision},
  author={Qing Dai and Sharon Moshitch-Moshkovitz and Dadong Han and N. J. C. Kol and Ninette Amariglio and Gideon Rechavi and Dan Dominissini and Chuan He},
  journal={Nature Methods},
  year={2017},
  volume={14},
  pages={695-698}
}
The ribose of RNA nucleotides can be 2′-O-methylated (Nm). Despite advances in high-throughput detection, the inert chemical nature of Nm still limits sensitivity and precludes mapping in mRNA. We leveraged the differential reactivity of 2′-O-methylated and 2′-hydroxylated nucleosides to periodate oxidation to develop Nm-seq, a sensitive method for transcriptome-wide mapping of Nm with base precision. Nm-seq uncovered thousands of Nm sites in human mRNA with features suggesting functional roles… CONTINUE READING
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