Nitrogenase switch-off by ammonium ions in Azospirillum brasilense requires the GlnB nitrogen signal-transducing protein.

@article{Klassen2005NitrogenaseSB,
  title={Nitrogenase switch-off by ammonium ions in Azospirillum brasilense requires the GlnB nitrogen signal-transducing protein.},
  author={Giseli Klassen and Emanuel Maltempi de Souza and Marshall Geoffrey Yates and Liu Un Rigo and Roberta M Costa and Juliana Inaba and F{\'a}bio de Oliveira Pedrosa},
  journal={Applied and environmental microbiology},
  year={2005},
  volume={71 9},
  pages={5637-41}
}
Nitrogenase activity in several diazotrophs is switched off by ammonium and reactivated after consumption. The signaling pathway to this system in Azospirillum brasilense is not understood. We show that ammonium-dependent switch-off through ADP-ribosylation of Fe protein was partial in a glnB mutant of A. brasilense but absent in a glnB glnZ double mutant. Triggering of inactivation by anaerobic conditions was not affected in either mutant. The results suggest that glnB is necessary for full… CONTINUE READING

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