Nitrogenase activity in a transfilter culture of rhizobia with a non-leguminous plant callus culture: Transfer of fixed15N2 from bacteria toPortulaca callus

Abstract

A transfilter culture of the non-leguminous plantPortulaca grandiflora var. JR andRhizobium sp. ‘cowpea’ 32H1 was established. Using15N2-analysis we demonstrated that15N-containing substances produced by the bacteria passed through the membrane and15N was enriched in the plant cells. 
DOI: 10.1007/BF01960111

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