• Biology, Medicine
  • Published in
    Canadian journal of…
    1992
  • DOI:10.1139/m92-049

Nitrogenase activity and growth of Frankia in batch and continuous culture.

@article{Harris1992NitrogenaseAA,
  title={Nitrogenase activity and growth of Frankia in batch and continuous culture.},
  author={Steven Lucas Harris and William Silvester},
  journal={Canadian journal of microbiology},
  year={1992},
  volume={38 4},
  pages={
          296-302
        }
}
Frankia grown in batch culture was unable to maintain a high rate of nitrogenase activity and, once a peak level was reached, activity rapidly declined. Addition of 5 mM carbon source of cultures or transfer to fresh medium was followed by brief recovery of nitrogenase activity. The extent of recovery decreased as additions or transfers were made to progressively older cultures. Daily addition of fresh medium (dilution rate = 0.125 day-1) allowed Frankia to be maintained in continuous… CONTINUE READING

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