Nitrogen-regulated ubiquitination of the Gap1 permease of Saccharomyces cerevisiae.

  title={Nitrogen-regulated ubiquitination of the Gap1 permease of Saccharomyces cerevisiae.},
  author={Jean Yves Springael and Bruno Andr{\'e}},
  journal={Molecular biology of the cell},
  volume={9 6},
Addition of ammonium ions to yeast cells growing on proline as the sole nitrogen source induces rapid inactivation and degradation of the general amino acid permease Gap1 through a process requiring the Npi1/Rsp5 ubiquitin (Ub) ligase. In this study, we show that NH4+ induces endocytosis of Gap1, which is then delivered into the vacuole where it is degraded. This down-regulation is accompanied by increased conversion of Gap1 to ubiquitinated forms. Ubiquitination and subsequent degradation of… CONTINUE READING


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