Nitrogen catabolite regulation of proline permease in Saccharomyces cerevisiae. Cloning of the PUT4 gene and study of PUT4 RNA levels in wild-type and mutant strains.

@article{Jauniaux1987NitrogenCR,
  title={Nitrogen catabolite regulation of proline permease in Saccharomyces cerevisiae. Cloning of the PUT4 gene and study of PUT4 RNA levels in wild-type and mutant strains.},
  author={J. C. Jauniaux and Micheline Vandenbol and St{\'e}phan Vissers and Kathy Broman and Marcelle Grenson},
  journal={European journal of biochemistry},
  year={1987},
  volume={164 3},
  pages={601-6}
}
The proline permease gene PUT4 has been cloned. Nitrogen-source regulation ('ammonia sensitivity') of this and at least two other amino-acid permeases is believed to occur at two distinct levels, i.e. permease synthesis and permease activity. Therefore, PUT4 transcription/messenger stability was examined in the ammonia- and proline-grown wild type as well as in mutant strains supposedly affected at only one or at both of these levels. We report transcript-level repression of proline permease… CONTINUE READING

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