Nitroavidin as a ligand for the surface capture and release of biotinylated proteins.

  title={Nitroavidin as a ligand for the surface capture and release of biotinylated proteins.},
  author={Jowell G. Bolivar and Steven A. Soper and Robin L. McCarley},
  journal={Analytical chemistry},
  volume={80 23},
Discussed here is the preparation, detailed purification, and evaluation of nitroavidin as a ligand for surface capture and release of biotinylated proteins. Avidin from chicken egg white was nitrated using dilute tetranitromethane solutions. UV-vis spectroscopy was used to show decreased binding of the biotin analogue, 2-(4'-hydroxyazobenzene)benzoic acid, HABA, to nitroavidin compared to binding of HABA to native avidin. From enzyme-linked immunosorbent assay (ELISA)-based assays of the… 
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