Nitro-L-arginine inteferes with the cadmium reduction of nitrate/griess reaction method of measuring nitric oxide production.

@article{Nithipatikom1996NitroLarginineIW,
  title={Nitro-L-arginine inteferes with the cadmium reduction of nitrate/griess reaction method of measuring nitric oxide production.},
  author={Kasem Nithipatikom and Phillip F. Pratt and William Campbell},
  journal={European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies},
  year={1996},
  volume={34 2},
  pages={133-7}
}
Nitro-L-arginine is used as an inhibitor of nitric oxide synthase in many biological Systems. Nitric oxide (NO) is unstable and degrades to nitrite NO(2)- and nitrate NO(3)-. The colorimetric reaction of N0(2)- with Griess reagent is commonly used to measure NO(2)-. NO(3)- may be measured as NO(2)- following reduction by cadmium or cadmium/copper. We found that bradykinin increased the formation of NO(2)- by bovine coronary endothelial cells. Nitro-L-arginine further increased the formation of… CONTINUE READING