Nitric oxide synthase expression in single hippocampal neurons.

@article{Chiang1994NitricOS,
  title={Nitric oxide synthase expression in single hippocampal neurons.},
  author={Lillian W Chiang and Felix Erich Schweizer and Richard W. Tsien and Howard F Schulman},
  journal={Brain research. Molecular brain research},
  year={1994},
  volume={27 1},
  pages={183-8}
}
The presence of nitric oxide synthase (NOS) in CA1 pyramidal cells of the rat hippocampus was demonstrated by single-cell PCR. NOS-specific primers were used to amplify mRNA isolated from single hippocampal neurons. The sequence of the major amplification-product obtained was identical to that of the constitutively expressed brain-isoform of NOS. These results confirm immunocytochemical data that NOS is present in CA1, and, therefore, nitric oxide could function as a retrograde messenger in… CONTINUE READING

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