Nitric oxide suppression of human hematopoiesis in vitro. Contribution to inhibitory action of interferon-gamma and tumor necrosis factor-alpha.

@article{Maciejewski1995NitricOS,
  title={Nitric oxide suppression of human hematopoiesis in vitro. Contribution to inhibitory action of interferon-gamma and tumor necrosis factor-alpha.},
  author={Jaroslaw Maciejewski and Carmine Selleri and Tsutomu Sato and Hyo Jin Cho and Larry K. Keefer and Carl Nathan and Neal S. Young},
  journal={The Journal of clinical investigation},
  year={1995},
  volume={96 2},
  pages={1085-92}
}
IFN-gamma and TNF-alpha, potent inhibitors of hematopoiesis, induce nitric oxide synthase (NOS) in various cell types. When normal human bone marrow (BM) or CD34+ cells were exposed to NO, inhibition of colony formation was dose dependent and direct. NO induced apoptosis in BM progenitors, as shown by electrophoretic detection of DNA degradation and deoxynucleotidyl transferase assay. Using PCR and immunoprecipitation, we found inducible NOS (iNOS) mRNA and iNOS protein in BM after stimulation… CONTINUE READING

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