Nitric oxide production in human macrophagic cells phagocytizing opsonized zymosan: direct characterization by measurement of the luminol dependent chemiluminescence.

@article{Gross1998NitricOP,
  title={Nitric oxide production in human macrophagic cells phagocytizing opsonized zymosan: direct characterization by measurement of the luminol dependent chemiluminescence.},
  author={Antoine Gross and Nathalie Dugas and S Spiesser and Ioannis Vouldoukis and Chantal Damais and J. P. Kolb and Bernard Dugas and Jacques Dornand},
  journal={Free radical research},
  year={1998},
  volume={28 2},
  pages={179-91}
}
When differentiated into mature macrophages by the combination of all-trans retinoic acid and 1,25-dihydroxyvitamin D3, the human promonocytic cell lines U937 and THP-1 expressed inducible nitric oxide synthase (iNOS) transcripts. During their differentiation, the cells acquired the capacity to produce not only superoxide anion (O2.-) but also nitric oxide (.NO) in response to IgG (or IgE)-opsonized zymosan. The inhibitors of the iNOS pathway, aminoguanidine and NG-monomethyl-L-arginine (L-NMMA… CONTINUE READING