Nitric oxide and nitric oxide-generating agents induce a reversible inactivation of protein kinase C activity and phorbol ester binding.

@article{Gopalakrishna1993NitricOA,
  title={Nitric oxide and nitric oxide-generating agents induce a reversible inactivation of protein kinase C activity and phorbol ester binding.},
  author={Rayudu Gopalakrishna and Zhang Hui Chen and Usha Gundimeda},
  journal={The Journal of biological chemistry},
  year={1993},
  volume={268 36},
  pages={27180-5}
}
Since S-nitrosylation of protein thiols is one of the cellular regulatory mechanisms induced by nitric oxide (NO), and since protein kinase C (PKC) has critical thiol residues which influence its kinase activity, we have determined whether NO could regulate this enzyme. Initial studies were carried out with purified PKC and the NO-generating agent S-nitrosocysteine. This agent decreased phosphotransferase activity of PKC in a Ca(2+)- and oxygen-dependent manner with an IC50 of 75 microM… CONTINUE READING

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