Nitric Oxide and Hydrogen Peroxide Mediate Wounding-Induced Freezing Tolerance through Modifications in Photosystem and Antioxidant System in Wheat

  title={Nitric Oxide and Hydrogen Peroxide Mediate Wounding-Induced Freezing Tolerance through Modifications in Photosystem and Antioxidant System in Wheat},
  author={Tong Si and Xiao Wang and Lin-Bo Wu and Chunzhao Zhao and Lini Zhang and Mei Huang and Jian Cai and Qin Zhou and Tingbo Dai and Jian-Kang Zhu and Dong Jiang},
  journal={Frontiers in Plant Science},
Mechanical wounding is a common stress caused by herbivores or manual and natural manipulations, whereas its roles in acclimation response to a wide spectrum of abiotic stresses remain unclear. The present work showed that local mechanical wounding enhanced freezing tolerance in untreated systemic leaves of wheat plants (Triticum aestivum L.), and meanwhile the signal molecules hydrogen peroxide (H2O2) and nitric oxide (NO) were accumulated systemically. Pharmacological study showed that… 

Figures from this paper

The Role of Hydrogen Peroxide in Mediating the Mechanical Wounding-Induced Freezing Tolerance in Wheat
Results support the hypothesis that local mechanical wounding-induced SWR in newly occurred leaves is largely attributed to RBOH-dependent H2O2 production, which may subsequently induce freezing tolerance in wheat plants and have a potential application to reduce the yield losses of wheat under late spring freezing conditions.
Hydrogen Peroxide and Abscisic Acid Mediate Salicylic Acid-Induced Freezing Tolerance in Wheat
It is demonstrated that endogenous H2O2 and ABA signaling may form a positive feedback loop to mediate SA-induced freezing tolerance in wheat.
Roles of Nitric Oxide in Conferring Multiple Abiotic Stress Tolerance in Plants and Crosstalk with Other Plant Growth Regulators
The biosynthesis and metabolism of NO and its crosstalk with numerous other signaling compounds are pointed out and recent cellular and molecular advances in NO signaling cross-talk under abiotic stress adaptations also have been discussed.
Nitric Oxide and Its Interaction with Hydrogen Peroxide Enhance Plant Tolerance to Low Temperatures by Improving the Efficiency of the Calvin Cycle and the Ascorbate–Glutathione Cycle in Cucumber Seedlings
The present study was aimed to assess the effect of nitric oxide (NO) and the interaction of NO with hydrogen peroxide (H2O2) on plant tolerance to low temperatures in cucumber seedlings. Exogenous
Reactive Oxygen Species Generation, Scavenging and Signaling in Plant Defense Responses
This present chapter provides an update on ROS generation, scavenging, and redox signaling in the context of plant abiotic stress tolerance, and identification and characterization of new targets and key regulator ROS-signaling transduction pathways which may provide excellent future candidates for breeding/engineering stress-resilient crop plants.
Proteomic Analysis of MeJa-Induced Defense Responses in Rice against Wounding
The results add novel insight into the molecular actors and physiological mechanisms orchestrated by MeJA in enhancing rice plants defenses after wounding and show that at the phenotypic level MeJA protects plants from oxidative stress and photosynthetic damage induced by wounding.
Resistance of Fritillaria imperialis to freezing stress through gene expression, osmotic adjustment and antioxidants
The findings suggest that Fritillaria efficiently tolerated freezing stress through induction of signalling mechanisms and overexpression of cold stress-responsive genes, and prevention of cold-induced water stress, oxidative stress and photosynthetic damage.


Nitric oxide activates superoxide dismutase and ascorbate peroxidase to repress the cell death induced by wounding
The results indicate that NO may regulate H2O2 generation to affect cell death in wounded plants, and Copper/Zinc superoxide dismutases could be enhanced by NO.
Hydrogen Peroxide Acts as a Second Messenger for the Induction of Defense Genes in Tomato Plants in Response to Wounding, Systemin, and Methyl Jasmonate
The cumulative results suggest that active oxygen species are generated near cell walls of vascular bundle cells by oligogalacturonide fragments produced by wound-inducible polygalacturonase and that the resulting H2O2 acts as a second messenger for the activation of defense genes in mesophyll cells.
Hydrogen peroxide is generated systemically in plant leaves by wounding and systemin via the octadecanoid pathway.
  • M. Orozco-Cárdenas, C. Ryan
  • Environmental Science
    Proceedings of the National Academy of Sciences of the United States of America
  • 1999
The cumulative data suggest that systemic wound signals that induce PG activity and H2O2 are widespread in the plant kingdom and that the response may be associated with the defense of plants against both herbivores and pathogens.
Nitrate reductase-mediated early nitric oxide burst alleviates oxidative damage induced by aluminum through enhancement of antioxidant defenses in roots of wheat (Triticum aestivum).
It is suggested that an NR-mediated early NO burst plays an important role in Al resistance of wheat through modulating enhanced antioxidant defense to adapt to Al stress.
Systemic Induction of NO-, Redox-, and cGMP Signaling in the Pumpkin Extrafascicular Phloem upon Local Leaf Wounding
Findings suggest that NO-dependent S-nitrosylation turned into peroxynitrite-mediated protein nitration upon a stress-induced redox shift probably involving the accumulation of reactive oxygen species within the cucurbit EFP.
ABA-induced NO generation and stomatal closure in Arabidopsis are dependent on H2 O2 synthesis.
Physiological and genetic data demonstrate a strong inter-relationship between ABA, endogenous H2 O2 and NO-induced stomatal closure and in the NADPH oxidase deficient double mutant atrbohD/F, NO synthesis and stomatic closure to ABA are severely reduced, indicating that endogenous H 2 O2 production induced by ABA is required for NO synthesis.
Nitric Oxide Negatively Modulates Wound Signaling in Tomato Plants1
The results suggest that NO may have a role in down-regulating the expression of wound-inducible defense genes during pathogenesis, and appears to be interacting directly with the signaling pathway downstream from JA synthesis, upstream of H2O2synthesis.
Nitric Reductase-Dependent Nitric Oxide Production Is Involved in Cold Acclimation and Freezing Tolerance in Arabidopsis1[W][OA]
NR-dependent NO production plays an important role in cold acclimation-induced increase in freezing tolerance by modulating Pro accumulation in Arabidopsis, and Pharmacological studies using NR inhibitor, NO scavenger, and NO donor showed that NR- dependent NO level was positively correlated with freezing tolerance.
Control of Ascorbate Peroxidase 2 expression by hydrogen peroxide and leaf water status during excess light stress reveals a functional organisation of Arabidopsis leaves.
Imaging of chlorophyll fluorescence and the production of reactive oxygen species indicated that APX2 expression followed a localised increase in hydrogen peroxide resulting from photosynthetic electron transport in the bundle sheath cells, and exposure of ABA-insensitive mutants to excess light resulted in reduced levels of APx2 expression and confirmed a role for ABA in the signalling pathway.
Mechanical wounding induces a nitrosative stress by down-regulation of GSNO reductase and an increase in S-nitrosothiols in sunflower (Helianthus annuus) seedlings
It is proposed that mechanical wounding triggers the accumulation of SNOs, specifically GSNO, due to a down-regulation of GSNOR activity, while NO2-Tyr increases, and a process of nitrosative stress is induced in sunflower seedlings and SNOs constitute a new wound signal in plants.