Nitrate signaling by the regulatory gene NIT2 in Chlamydomonas.

  title={Nitrate signaling by the regulatory gene NIT2 in Chlamydomonas.},
  author={Antonio Camargo and Angel Llamas and Rogene A. Schnell and Jos{\'e} Javier Higuera and David Gonz{\'a}lez-Ballester and Paul A Lefebvre and Emilio Fern{\'a}ndez and A. Camacho – Galv{\'a}n},
  journal={The Plant cell},
  volume={19 11},
Positive signaling by nitrate in its assimilation pathway has been studied in Chlamydomonas reinhardtii. Among >34,000 lines generated by plasmid insertion, 10 mutants were unable to activate nitrate reductase (NIA1) gene expression and had a Nit(-) (no growth in nitrate) phenotype. Each of these 10 lines was mutated in the nitrate assimilation-specific regulatory gene NIT2. The complete NIT2 cDNA sequence was obtained, and its deduced amino acid sequence revealed GAF, Gln-rich, Leu zipper, and… CONTINUE READING


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