Nipple Aspirate Fluid Hormone Concentrations and Breast Cancer Risk

  title={Nipple Aspirate Fluid Hormone Concentrations and Breast Cancer Risk},
  author={R. Chatterton and R. Heinz and A. Fought and D. Ivancic and C. Shappell and Subhashini Allu and S. Gapstur and D. Scholtens and P. Gann and Seema A. Khan},
  journal={Hormones and Cancer},
Prior reports identify higher serum concentrations of estrogens and androgens as risk factors for breast cancer, but steroids in nipple aspirate fluid (NAF) may be more related to risk. Incident breast cancer cases and mammography controls were recruited. Sex steroids were measured in NAF from the unaffected breasts of cases and one breast of controls. Menopausal status and menstrual cycle phase were determined. NAF steroids were purified by HPLC and quantified by immunoassays. Conditional… Expand
Protein Biomarkers for Breast Cancer Risk Are Specifically Correlated with Local Steroid Hormones in Nipple Aspirate Fluid
NAF proteins were more strongly related to local hormone levels than to systematic hormone levels, suggesting that these steroids may contribute to breast cancer risk through different mechanisms. Expand
Breast Hormone Concentrations in Random Fine-Needle Aspirates of Healthy Women Associate with Cytological Atypia and Gene Methylation
The authors' data indicate that future larger studies of breast steroid hormones along with other parameters are feasible, and significant associations of breast androgen concentrations with breast density and gene methylation warrant future study. Expand
High-Throughput Proteomic Profiling of Nipple Aspirate Fluid from Breast Cancer Patients Compared with Non-Cancer Controls: A Step Closer to Clinical Feasibility
Findings show 1D LC-MS/MS can quantify changes reflected in the NAF proteome associated with breast cancer development, and perturbations of the most abundant NAF proteins in BC patients compared with non-cancer controls could be monitored and validated in larger groups. Expand
Non-Invasive Biomarkers for Early Detection of Breast Cancer
Circulating carcinoma antigens, circulating tumor cells, circulating cell-free tumor nucleic acids (DNA or RNA), circulating microRNAs, and circulating extracellular vesicles in the peripheral blood, nipple aspirate fluid, sweat, urine, and tears, as well as volatile organic compounds in the breath, have emerged as potential non-invasive diagnostic biomarkers to supplement current clinical approaches to earlier detection of breast cancer. Expand
A randomized controlled trial of metformin in women with components of metabolic syndrome: intervention feasibility and effects on adiposity and breast density
Metformin intervention resulted in favorable changes in anthropometric measures of adiposity and a borderline decrease in non-dense breast volume in women with metabolic dysregulation in a Phase II double-blind, randomized, placebo-controlled trial of metformin in overweight/obese premenopausal women. Expand
Cytological Examination of Breast Fluids for Cancer Screening – Oxford Clinical Policy
  • 2021
Table of
  • 2014
Table of


Estrogen and Progesterone Levels in Nipple Aspirate Fluid of Healthy Premenopausal Women: Relationship to Steroid Precursors and Response Proteins
The low correlation between estrogen levels in NAF and those in serum or saliva suggests that the degree of association between estrogen or its androgen precursor levels and risk of breast cancer observed in epidemiologic studies using serum estimates might be highly attenuated. Expand
Methodological considerations in estrogen assays of breast fluid and breast tissue
The similarity of levels between breasts and between ducts suggests that estimates of estrogen exposure does not require multiple samples, however, unavailability of fluid may require rFNA in some cases. Expand
Serum sex hormone levels are related to breast cancer risk in postmenopausal women.
The results lend considerable support to the hypothesis that serum concentrations of estrogens and androgens are related to the subsequent diagnosis of breast cancer in postmenopausal women. Expand
Serum levels of sex hormones and breast cancer risk in premenopausal women: a case–control study (USA)
In summary, it did not find a convincing association between serum SHBG, estradiol, testosterone or androstenedione and premenopausal breast cancer risk and observed differences between cases and controls subjects in serum levels of DHEA and luteal phase progesterone should be investigated further in large prospective studies. Expand
Endogenous steroid hormone concentrations and risk of breast cancer among premenopausal women.
Levels of circulating estrogens and androgens may be important in the etiology of premenopausal breast cancer. Expand
Breast Ductal Lavage for Assessment of Breast Cancer Biomarkers
The fact that DLF E2 does not reflect the high serum concentrations of E2 during tamoxifen treatment indicates that breast concentrations of estradiol may be under feedback control, and unlike studies of low risk populations, progesterone concentrations were not significantly less in postmenopausal than in premenopausal women. Expand
Serum Sex Steroids in Premenopausal Women and Breast Cancer Risk Within the European Prospective Investigation into Cancer and Nutrition (EPIC)
A case-control study nested within the European Prospective Investigation into Cancer and Nutrition cohort to examine associations among premenopausal serum concentrations of sex steroids and subsequent breast cancer risk found no statistically significant association with serum levels. Expand
Premenopausal serum sex hormone levels in relation to breast cancer risk, overall and by hormone receptor status—Results from the EPIC cohort
Higher premenopausal circulating testosterone levels are associated with an increased risk of developing breast cancer, but do not show a significant association of estradiol or progesterone with breast cancer risk, overall, by menstrual cycle phase or by tumor receptor status, although a possible risk increase with higherEstradiol levels for tumors diagnosed before age 50 was seen. Expand
Circulating estrogens and estrogens within the breast among postmenopausal BRCA1/2 mutation carriers
It is demonstrated that circulating PE and EM are strongly and positively correlated with tissue-specific PE andEM measured in NAF and DLS among postmenopausal BRCA1/2 mutation carriers, and future etiologic studies could utilize the more readily obtainable serum hormone levels as a reliable surrogate measure of exposure at the tissue level. Expand
Plasma sex steroid hormone levels and risk of breast cancer in postmenopausal women.
The data, in conjunction with past epidemiologic and animal studies, provide strong evidence for a causal relationship between postmenopausal estrogen levels and the risk of breast cancer. Expand