Nipah virus glycoprotein: production in baculovirus and application in diagnosis.

@article{Eshaghi2004NipahVG,
  title={Nipah virus glycoprotein: production in baculovirus and application in diagnosis.},
  author={Majid Eshaghi and Wen Siang Tan and Taznim Begam Mohd Mohidin and Khatijah Yusoff},
  journal={Virus research},
  year={2004},
  volume={106 1},
  pages={
          71-6
        }
}
A method for serological diagnosis of Nipah virus (NiV) is described. DNA encoding truncated G protein of NiV was cloned into the pFastBac HT vector, and the fusion protein to His-tag was expressed in insect cells by recombinant baculovirus. The resulting His-G recombinant fusion protein was purified by affinity chromatography and used as the coating antigen for serological testing by indirect enzyme-linked immunosorbant assay (ELISA). When tested against a panel of swine serum samples, the… CONTINUE READING