Ninhydrin reaction on thiol-reactive solid and its potential for the quantitation of D-penicillamine.


While aminothiols produce weak purple-colored reactions with ninhydrin, we demonstrate for the first time that this color could be intensely developed. Using a D-penicillamine paradigm, adsorption of this compound via a disulfide bond onto thiol-reactive solid prior to ninhydrin reaction allowed spectrophotometrical monitoring of the supernatant at 570 nm… (More)
DOI: 10.1016/j.talanta.2010.01.016