Nineteen baculovirus open reading frames, including LEF-12, support late gene expression.

@article{Rapp1998NineteenBO,
  title={Nineteen baculovirus open reading frames, including LEF-12, support late gene expression.},
  author={Jeffrey C. Rapp and Joyce A Wilson and Lois K. Miller},
  journal={Journal of virology},
  year={1998},
  volume={72 12},
  pages={10197-206}
}
A set of 18 plasmid subclones of the Autographa californica nuclear polyhedrosis virus genome, each containing an identified late expression factor gene (lef), supports expression from a late viral promoter in transient expression assays in the SF-21 cell line derived from Spodoptera frugiperda. We have constructed a further set of plasmids in which each lef open reading frame (ORF) is controlled by the Drosophila melanogaster heat shock protein 70 (hsp70) promoter and epitope tagged. Failure… CONTINUE READING
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