Nimesulide binding site in the B0AT1 (SLC6A19) amino acid transporter. Mechanism of inhibition revealed by proteoliposome transport assay and molecular modelling.

@article{Pochini2014NimesulideBS,
  title={Nimesulide binding site in the B0AT1 (SLC6A19) amino acid transporter. Mechanism of inhibition revealed by proteoliposome transport assay and molecular modelling.},
  author={Lorena Pochini and Angela Seidita and Cristina Sensi and Mariafrancesca Scalise and Ivano Eberini and Cesare Indiveri},
  journal={Biochemical pharmacology},
  year={2014},
  volume={89 3},
  pages={422-30}
}
The effect of pharmaceutical compounds on the rat kidney B0AT1 transporter in proteoliposomes has been screened. To this aim, inhibition of the transport activity by the different compounds was measured on Na(+)-[(3)H]glutamine co-transport in the presence of membrane potential positive outside. Most of the tested drugs had no effect on the transport activity. Some compounds exhibited inhibitory effects from 5 to 88% at concentration of 300μM. Among the tested compounds, only the anti… CONTINUE READING