Nile red as a probe for lipid-storage products in benthic copepods

  title={Nile red as a probe for lipid-storage products in benthic copepods},
  author={Kevin and R. and Carman},
Nile red (NR) is a hydrophobic fluorophore that binds specifically to neutral lipids. We examined the usefulness of NR as a probe for detecting neutral-llpid-storage products in benthc (harpacticoid) copepods. Following a brief (30 min) exposure to NR, lipid droplets, which fluoresce a bright yellow/orange, were clearly visible under epifluorescent illumination. Analysis of fluorescent images was used to determine relative lipid concentrations. The NR procedure was used on copepods subjected to… CONTINUE READING


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