Nile Red is a fluorescent allosteric substrate of cytochrome P450 3A4.

  title={Nile Red is a fluorescent allosteric substrate of cytochrome P450 3A4.},
  author={Jed N. Lampe and Cristina G{\'o}mez Fern{\'a}ndez and Abhinav Nath and William M Atkins},
  volume={47 2},
Cytochrome P450 3A4 (CYP3A4) plays a critical role in the metabolism of many drugs. CYP3A4 exhibits extraordinary substrate promiscuity and unusual allosteric kinetics. In addition, many CYPs catalyze sequential oxidations on a single substrate, but in most cases, mechanistic details of these processes are not well-established. As a result, in vivo clearance of many drugs and their metabolites is difficult to predict on the basis of the complex in vitro kinetics, and new in vitro probes are… CONTINUE READING


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Binding of 7methoxy4( aminomethyl )coumarin to wild - type and W 128 F mutant cytochrome P 450 2 D 6 studied by time - resolved fluorescence spectroscopy

A. Stortelder, P. H. Keizers, +6 authors G. Van der Zwan
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