Nile Red binding to HepG2 cells: an improved assay for in vitro studies of hepatosteatosis.

@article{McMillian2001NileRB,
  title={Nile Red binding to HepG2 cells: an improved assay for in vitro studies of hepatosteatosis.},
  author={Michael K McMillian and Elfrida R. Grant and Zhi-hui Zhong and J. Brandon Parker and Linhao Li and Robert A. Zivin and Michael E. Burczynski and Mark D Johnson},
  journal={In vitro & molecular toxicology},
  year={2001},
  volume={14 3},
  pages={
          177-90
        }
}
Nile Red is a fluorescent dye used extensively to study fat accumulation in many types of cells; unfortunately protocols that work well for most cells are not effective for studying drug-induced lipid accumulation in cultured liver cells and hepatocyte-derived cell lines. Using human hepatoma (HepG2) cells, we have developed a simple Nile Red binding assay as a screen for steatosis-inducing compounds. Increases in Nile Red binding in response to known hepatotoxic compounds were observed after… CONTINUE READING

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