Nicotinamide-adenine dinucleotide regulates muscarinic receptor-coupled K+ (M) channels in rodent NG108-15 cells.

@article{Higashida1995NicotinamideadenineDR,
  title={Nicotinamide-adenine dinucleotide regulates muscarinic receptor-coupled K+ (M) channels in rodent NG108-15 cells.},
  author={Haruhiro Higashida and Jeffrey Robbins and Anastasia Egorova and Mami Noda and M Taketo and Nobuto Ishizaka and Shin Takasawa and H T S Okamoto and David Brown},
  journal={The Journal of physiology},
  year={1995},
  volume={482 ( Pt 2)},
  pages={317-23}
}
1. The possible role of nicotinamide-adenine dinucleotide (NAD+) and cyclic adenosine diphosphate ribose (cADPR) as regulators of M-type K+ currents (IK(M)) has been studied in whole-cell patch-clamped NG108-15 mouse neuroblastoma x rat glioma cells that had been transformed to express m1 muscarinic acetylcholine receptors (mAChRs). 2. Pre-incubation of NG108-15 cells for 6-8 h with streptozotocin (2-5 mM) reduced NAD+ levels by 40-50%. Nicotinamide (2-5 mM) increased NAD+ levels and prevented… CONTINUE READING