Nicotianamine synthase 2 localizes to the vesicles of iron-deficient rice roots, and its mutation in the YXXφ or LL motif causes the disruption of vesicle formation or movement in rice.

@article{Nozoye2014NicotianamineS2,
  title={Nicotianamine synthase 2 localizes to the vesicles of iron-deficient rice roots, and its mutation in the YXXφ or LL motif causes the disruption of vesicle formation or movement in rice.},
  author={Tomoko Nozoye and Seiji Nagasaka and Khurram Bashir and Michiko Takahashi and Takanori Kobayashi and Hiromi Nakanishi and Naoko K. Nishizawa},
  journal={The Plant journal : for cell and molecular biology},
  year={2014},
  volume={77 2},
  pages={246-60}
}
Graminaceous plants release mugineic acid family phytosiderophores (MAs) to acquire iron from the soil. Here, we show that deoxymugineic acid (DMA) secretion from rice roots fluctuates throughout the day, and that vesicles accumulate in roots before MAs secretion. We developed transgenic rice plants that express rice nicotianamine (NA) synthase (NAS) 2 (OsNAS2) fused to synthetic green fluorescent protein (sGFP) under the control of its own promoter. In root cells, OsNAS2-sGFP fluorescence was… CONTINUE READING