Nickel-[iron-sulfur]-selenium-containing hydrogenases from Desulfovibrio baculatus (DSM 1743). Redox centers and catalytic properties.

@article{Teixeira1987Nickelironsulfurseleniumcontainin,
  title={Nickel-[iron-sulfur]-selenium-containing hydrogenases from Desulfovibrio baculatus (DSM 1743). Redox centers and catalytic properties.},
  author={Miguel Teixeira and Guy D. Fauque and Isabel Moura and P A Lespinat and Y M Berlier and B C Prickril and Harry D. Peck and Ant{\'o}nio V. Xavier and Jean Le Gall and Jos{\'e} J. G. Moura},
  journal={European journal of biochemistry},
  year={1987},
  volume={167 1},
  pages={47-58}
}
The hydrogenase from Desulfovibrio baculatus (DSM 1743) was purified from each of three different fractions: soluble periplasmic (wash), soluble cytoplasmic (cell disruption) and membrane-bound (detergent solubilization). Plasma-emission metal analysis detected in all three fractions the presence of iron plus nickel and selenium in equimolecular amounts. These hydrogenases were shown to be composed of two non-identical subunits and were distinct with respect to their spectroscopic properties… CONTINUE READING

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