Niche-independent high-purity cultures of Lgr5+ intestinal stem cells and their progeny

  title={Niche-independent high-purity cultures of Lgr5+ intestinal stem cells and their progeny},
  author={X. Yin and H. Farin and J. V. Es and H. Clevers and R. Langer and J. Karp},
  journal={Nature Methods},
  • X. Yin, H. Farin, +3 authors J. Karp
  • Published 2014
  • Biology, Medicine
  • Nature Methods
  • Although Lgr5+ intestinal stem cells have been expanded in vitro as organoids, homogeneous culture of these cells has not been possible thus far. Here we show that two small molecules, CHIR99021 and valproic acid, synergistically maintain self-renewal of mouse Lgr5+ intestinal stem cells, resulting in nearly homogeneous cultures. The colony-forming efficiency of cells from these cultures is ∼100-fold greater than that of cells cultured in the absence of CHIR99021 and valproic acid, and… CONTINUE READING
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