Ni(ii)-modified solid substrates as a platform to adsorb His-tag proteins.

@article{Valenti2013NiiimodifiedSS,
  title={Ni(ii)-modified solid substrates as a platform to adsorb His-tag proteins.},
  author={Laura E. Valenti and Vitor L Martins and Elisa Herrera and Roberto Manuel Torresi and Carla E. Giacomelli},
  journal={Journal of materials chemistry. B},
  year={2013},
  volume={1 38},
  pages={
          4921-4931
        }
}
This work investigated a simple and versatile modification to a solid substrate to develop electrochemical bio-recognition platforms based on bio-affinity interactions between histidine (His)-tagged proteins and Ni(ii) surface sites. Carboxylate (COO)-functionalized substrates were prepared in multiple steps, initiated with an amino-terminated self-assembled monolayer (SAM) on polycrystalline gold. Surface enhanced Raman spectroscopy (SERS), quartz crystal microbalance with dissipation… Expand
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