Ni/NiO core/shell nanoparticles for selective binding and magnetic separation of histidine-tagged proteins.

@article{Lee2006NiNiOCN,
  title={Ni/NiO core/shell nanoparticles for selective binding and magnetic separation of histidine-tagged proteins.},
  author={In Su Lee and Nohyun Lee and Jongnam Park and Byung Hyo Kim and Yong Yi and Taeuk Kim and Tae Kook Kim and In Hwan Lee and Seung R Paik and Taeghwan Hyeon},
  journal={Journal of the American Chemical Society},
  year={2006},
  volume={128 33},
  pages={10658-9}
}
Ni/NiO core/shell nanoparticles having high affinity with polyhistidine were synthesized by decomposition of a Ni surfactant complex followed by air oxidation. Ni/NiO nanoparticles showed selective and efficient binding to histidine-tagged proteins and easy separation by using a magnet. These provided a more convenient way to efficient purification of histidine-tagged proteins compared with the conventional Ni-NTA complex-bound resins and microbeads. 

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