Next-generation sequencing transforms today's biology

@article{Schuster2008NextgenerationST,
  title={Next-generation sequencing transforms today's biology},
  author={Stephan C. Schuster},
  journal={Nature Methods},
  year={2008},
  volume={5},
  pages={16-18}
}
  • S. Schuster
  • Published 2008
  • Biology, Medicine
  • Nature Methods
A new generation of non-Sanger-based sequencing technologies has delivered on its promise of sequencing DNA at unprecedented speed, thereby enabling impressive scientific achievements and novel biological applications. However, before stepping into the limelight, next-generation sequencing had to overcome the inertia of a field that relied on Sanger-sequencing for 30 years. 

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References

SHOWING 1-10 OF 20 REFERENCES
A Sanger/pyrosequencing hybrid approach for the generation of high-quality draft assemblies of marine microbial genomes.
TLDR
This study evaluated the utility and cost-effectiveness of a hybrid sequencing approach using 3730xl Sanger data and 454 data to generate higher-quality lower-cost assemblies of microbial genomes compared to current Sanger sequencing strategies alone. Expand
Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome
We describe a DNA sequencing technology in which a commonly available, inexpensive epifluorescence microscope is converted to rapid nonelectrophoretic DNA sequencing automation. We apply thisExpand
Genome sequencing in microfabricated high-density picolitre reactors
TLDR
A scalable, highly parallel sequencing system with raw throughput significantly greater than that of state-of-the-art capillary electrophoresis instruments with 96% coverage at 99.96% accuracy in one run of the machine is described. Expand
DNA sequencing with chain-terminating inhibitors.
TLDR
A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. Expand
A new method for sequencing DNA.
  • A. Maxam, W. Gilbert
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1977
TLDR
Reactions that cleave DNA preferentially at guanines, at adenines,At cytosines and thymines equally, and at cytosine alone are described. Expand
Whole-Genome Shotgun Sequencing of Mitochondria from Ancient Hair Shafts
TLDR
The observed levels of damage-derived sequencing errors were lower than those observed in previously published frozen bone samples, even though one of the specimens was >50,000 14C years old and another had been stored for 200 years at room temperature. Expand
Metagenomics to Paleogenomics: Large-Scale Sequencing of Mammoth DNA
TLDR
The high percentage of endogenous DNA recoverable from this single mammoth would allow for completion of its genome, unleashing the field of paleogenomics. Expand
Nucleotide sequence of bacteriophage φX174 DNA
TLDR
The sequence identifies many of the features responsible for the production of the proteins of the nine known genes of the organism, including initiation and termination sites for the proteins and RNAs. Expand
Comprehensive mutation identification in an evolved bacterial cooperator and its cheating ancestor.
TLDR
In insight into the genetic basis of two large adaptive transitions in a social bacterium, a single mutation responsible for the restoration of development in strain PX was identified, whereas 14 mutations occurred during the prior phase of experimental evolution. Expand
Analysis of one million base pairs of Neanderthal DNA
TLDR
A 38,000-year-old Neanderthal fossil that is exceptionally free of contamination from modern human DNA is identified and it is revealed that modern human and Neanderthal DNA sequences diverged on average about 500,000 years ago. Expand
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