Next-generation sequencing transforms today's biology

  title={Next-generation sequencing transforms today's biology},
  author={Stephan C. Schuster},
  journal={Nature Methods},
A new generation of non-Sanger-based sequencing technologies has delivered on its promise of sequencing DNA at unprecedented speed, thereby enabling impressive scientific achievements and novel biological applications. However, before stepping into the limelight, next-generation sequencing had to overcome the inertia of a field that relied on Sanger-sequencing for 30 years. 

Application of 'next-generation' sequencing technologies to microbial genetics

The capabilities of high-throughput sequencing technologies are reviewed and the many options for getting useful information from the data are discussed.

Challenges in the Next-Generation Sequencing Field

This chapter compares next-generation sequencing with the traditional Sanger method to demonstrate the remarkable recent developments in relation to improved speed, throughput, and accuracy.

Next-generation sequencing: applications beyond genomes

A snapshot of these exciting new approaches to understanding the properties and functions of genomes is provided, given that sequencing-based assays may increasingly supersede microarray-basedAssays, to compare and contrast data obtained from these distinct approaches.

First- and Next-Generations Sequencing Methods

This chapter provides an overview of the majority of sequencing methods including techniques from the first- and next-generation of DNA sequencing.

Integrating Next Generation Sequencing, Bioinformatics and Cytogenomics in the Study of Brazilian Mammals

Since the discovery of the DNA double helix, understanding the complexity and diversity of genomes has been a major focus of genetics, including medical and evolutionary research. Sequencing methods

Development of Next Generation Sequencing Protocols for dsRNA Viruses

Next-generation sequencing methodologies is now changing the way that the authors understand viruses, particularly in the areas of genome sequencing, evolution, ecology, discovery and transcriptomics, and expect new challenges to understand the role of newly discovered viral diversity in both disease and health.

Whole Genome Sequencing in the Clinical Laboratory

The opportunities and challenges of developing a MPS protocol for whole genome sequencing within a clinical laboratory as well as its potential applications for clinical use are discussed.

Next generation sequencing: chemistry, technology and applications.

  • P. Hui
  • Medicine, Biology
    Topics in current chemistry
  • 2014
High-throughput next generation sequencing has been quickly adapted into many aspects of biomedical research and begun to engage with the clinical practice, but significant huddles need to be overcome before an ultimate application of NGS in genomic medicine can be practical and fruitful.

The emergence of nanopores in next-generation sequencing

This review summarizes past, present, and future DNA sequencing techniques, which are realized by nanopore approaches such as those pursued by Oxford Nanopore Technologies.

Evaluation of next-generation sequencing software in mapping and assembly

An overview of the challenges that these novel technologies meet are presented and various bioinformatics attempts on mapping and assembly for problem solving are illustrated and the performance of several programs in these two fields are compared.



A Sanger/pyrosequencing hybrid approach for the generation of high-quality draft assemblies of marine microbial genomes.

This study evaluated the utility and cost-effectiveness of a hybrid sequencing approach using 3730xl Sanger data and 454 data to generate higher-quality lower-cost assemblies of microbial genomes compared to current Sanger sequencing strategies alone.

Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome

We describe a DNA sequencing technology in which a commonly available, inexpensive epifluorescence microscope is converted to rapid nonelectrophoretic DNA sequencing automation. We apply this

Genome sequencing in microfabricated high-density picolitre reactors

A scalable, highly parallel sequencing system with raw throughput significantly greater than that of state-of-the-art capillary electrophoresis instruments with 96% coverage at 99.96% accuracy in one run of the machine is described.

A new method for sequencing DNA.

  • A. MaxamW. Gilbert
  • Biology, Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 1977
Reactions that cleave DNA preferentially at guanines, at adenines,At cytosines and thymines equally, and at cytosine alone are described.

Metagenomics to Paleogenomics: Large-Scale Sequencing of Mammoth DNA

The high percentage of endogenous DNA recoverable from this single mammoth would allow for completion of its genome, unleashing the field of paleogenomics.

Comprehensive mutation identification in an evolved bacterial cooperator and its cheating ancestor.

In insight into the genetic basis of two large adaptive transitions in a social bacterium, a single mutation responsible for the restoration of development in strain PX was identified, whereas 14 mutations occurred during the prior phase of experimental evolution.

Analysis of one million base pairs of Neanderthal DNA

A 38,000-year-old Neanderthal fossil that is exceptionally free of contamination from modern human DNA is identified and it is revealed that modern human and Neanderthal DNA sequences diverged on average about 500,000 years ago.

Sequencing and Analysis of Neanderthal Genomic DNA

The characterization of Neanderthals from a new perspective is described, based on the development of a Neanderthal metagenomic library and its high-throughput sequencing and analysis, and the finding that the Neanderthal and human genomes are at least 99.5% identical is found.

Sequencing Medicago truncatula expressed sequenced tags using 454 Life Sciences technology

Due to the large number of reads afforded by the 454 DNA sequencing technology, it is effective in revealing the expression of transcripts from a broad range of GO categories and contains many rare transcripts in normalized cDNA libraries, although only a limited portion of their sequence is uncovered.