Next-generation ion torrent sequencing of drug resistance mutations in Mycobacterium tuberculosis strains.

@article{Daum2012NextgenerationIT,
  title={Next-generation ion torrent sequencing of drug resistance mutations in Mycobacterium tuberculosis strains.},
  author={Luke T. Daum and John D. Rodriguez and Sue A. Worthy and Nazir Ahmed Ismail and Shaheed Vally Omar and Andries William Dreyer and P. Bernard Fourie and Anwar Ahmed Hoosen and James P. Chambers and Gerald W. Fischer},
  journal={Journal of clinical microbiology},
  year={2012},
  volume={50 12},
  pages={3831-7}
}
A novel protocol for full-length Mycobacterium tuberculosis gene analysis of first- and second-line drug resistance was developed using the Ion Torrent Personal Genome Machine (PGM). Five genes-rpoB (rifampin), katG (isoniazid), pncA (pyrazinamide), gyrA (ofloxacin/fluoroquinolone), and rrs (aminoglycosides)-were amplified and sequenced, and results were compared to those obtained by genotypic Hain line probe assay (LPA) and phenotypic Bactec MGIT 960 analysis using 26 geographically diverse… CONTINUE READING
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