Next-generation genotyping of hypervariable loci in many individuals of a non-model species: technical and theoretical implications

@article{Grogan2016NextgenerationGO,
  title={Next-generation genotyping of hypervariable loci in many individuals of a non-model species: technical and theoretical implications},
  author={Kathleen E Grogan and Gwendolyn J McGinnis and Michelle L. Sauther and Frank P. Cuozzo and Christine M Drea},
  journal={BMC Genomics},
  year={2016},
  volume={17},
  pages={1-16}
}
Across species, diversity at the Major Histocompatibility Complex (MHC) is critical to disease resistance and population health; however, use of MHC diversity to quantify the genetic health of populations has been hampered by the extreme variation found in MHC genes. Next generation sequencing (NGS) technology generates sufficient data to genotype even the most diverse species, but workflows for distinguishing artifacts from alleles are still under development. We used NGS to evaluate the MHC… CONTINUE READING