Next-generation DNA sequencing

  title={Next-generation DNA sequencing},
  author={Jay A. Shendure and Hanlee P. Ji},
  journal={Nature Biotechnology},
DNA sequence represents a single format onto which a broad range of biological phenomena can be projected for high-throughput data collection. Over the past three years, massively parallel DNA sequencing platforms have become widely available, reducing the cost of DNA sequencing by over two orders of magnitude, and democratizing the field by putting the sequencing capacity of a major genome center in the hands of individual investigators. These new technologies are rapidly evolving, and near… 
Frontiers in DNA Sequencing: the (R)Evolution of Sequencing Technologies
An overview of the currently available sequencing platforms, as well as a preview of promising new technologies that may enter the market in the near future are given.
High‐throughput DNA sequencing – concepts and limitations
  • Martin Kircher, J. Kelso
  • Biology
    BioEssays : news and reviews in molecular, cellular and developmental biology
  • 2010
The relevant concepts and issues raised by the current high‐throughput DNA sequencing technologies are reviewed and compared and how future developments may overcome these limitations are analyzed.
The evolution of high-throughput sequencing technologies: from Sanger to single-molecule sequencing
Next-generation sequencing technologies are characterized by their ability to perform massively parallel sequencing of up to hundred millions of sequence reads, which has significantly increased the throughput of the sequencing data and reduced the cost quite substantially.
The expanding scope of DNA sequencing
In just seven years, next-generation technologies have reduced the cost and increased the speed of DNA sequencing by four orders of magnitude, and experiments requiring many millions of sequencing
Perspectives and challenges of emerging single-molecule DNA sequencing technologies.
The growing demand for analysis of the genomes of many species and cancers, for understanding the role of genetic variation among individuals in disease, and with the ultimate goal of deciphering
Landscape of next-generation sequencing technologies.
This Review concentrates on the technology behind the third- and fourth-generation sequencing methods: their challenges, current limitations, and tantalizing promise.
Third Generation DNA Sequencing Technologies
Third generation DNA sequencing technologies are shown to be capable of working with a lot of studies on whole genome of any organism, in less than a day, much less cost, complete, accurate and creating longer sequencing reads chains.
Next-Generation DNA Sequencing: Methodology and Application
Development of commercial sequencing devices is reviewed and presently commercially available very high-throughput DNA sequencing platforms, as well as techniques under development, are described and their applications discussed.
The Impact of Next-Generation Sequencing Technology on Bacterial Genomics
Next-generation sequencing is discussed and how it has been used to study a variety of areas from gene expression and protein-DNA interactions to bacterial community function and evolution, at the scale of whole bacterial genomes.
Accuracy of Next Generation Sequencing Platforms.
A Duplex Sequencing additionally exploits the fact that DNA is double-strand, with one strand reciprocally encoding the sequence information of its complement, and can eliminate nearly all sequencing errors.


Single-Molecule DNA Sequencing of a Viral Genome
An amplification-free method for determining the nucleotide sequence of more than 280,000 individual DNA molecules simultaneously is reported, which demonstrates a strategy for high-throughput low-cost resequencing.
Whole-genome re-sequencing.
  • D. Bentley
  • Biology, Engineering
    Current opinion in genetics & development
  • 2006
DNA sequencing: bench to bedside and beyond†
New ‘massively parallel’ sequencing methods are greatly increasing sequencing capacity, but further innovations are needed to achieve the ‘thousand dollar genome’ that many feel is prerequisite to personalized genomic medicine.
The complete genome of an individual by massively parallel DNA sequencing
This sequence was completed in two months at approximately one-hundredth of the cost of traditional capillary electrophoresis methods and demonstrated the acquisition of novel human sequence, including novel genes not previously identified by traditional genomic sequencing, which is the first genome sequenced by next-generation technologies.
Large-scale and automated DNA sequence determination.
New sequencing methodologies, fully automated instrumentation, and improvements in sequencing-related computational resources may render genome-size sequencing projects (100 Mb or larger) feasible during the next 5 to 10 years.
Rapid genome sequencing with short universal tiling probes
A DNA sequencing method based on hybridization of a universal panel of tiling probes that consumes only dilute solutions of the probes, resulting in reduced sequencing cost and substantially increased speed is described.
Advanced sequencing technologies: methods and goals
Various novel sequencing technologies are being developed, each aspiring to reduce costs to the point at which the genomes of individual humans could be sequenced as part of routine health care.
Sequence information can be obtained from single DNA molecules
These experiments show that one can study the activity of DNA polymerase at the single molecule level with single base resolution and a high degree of parallelization, thus providing the foundation for a practical single molecule sequencing technology.
Genome sequencing in microfabricated high-density picolitre reactors
A scalable, highly parallel sequencing system with raw throughput significantly greater than that of state-of-the-art capillary electrophoresis instruments with 96% coverage at 99.96% accuracy in one run of the machine is described.
Comparing whole genomes using DNA microarrays
Advances in microarray-based approaches have enabled the main forms of genomic variation to be detected using techniques that are readily performed in individual laboratories using simple experimental approaches.