Next-Generation in Situ Hybridization Chain Reaction: Higher Gain, Lower Cost, Greater Durability

  title={Next-Generation in Situ Hybridization Chain Reaction: Higher Gain, Lower Cost, Greater Durability},
  author={Harry M T Choi and Victor A. Beck and Niles A. Pierce},
  booktitle={ACS nano},
Hybridization chain reaction (HCR) provides multiplexed, isothermal, enzyme-free, molecular signal amplification in diverse settings. Within intact vertebrate embryos, where signal-to-background is at a premium, HCR in situ amplification enables simultaneous mapping of multiple target mRNAs, addressing a longstanding challenge in the biological sciences. With this approach, RNA probes complementary to mRNA targets trigger chain reactions in which metastable fluorophore-labeled RNA hairpins self… CONTINUE READING
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