Next-Generation Genotyping by Digital PCR to Detect and Quantify the BRAF V600E Mutation in Melanoma Biopsies.

@article{Lamy2015NextGenerationGB,
  title={Next-Generation Genotyping by Digital PCR to Detect and Quantify the BRAF V600E Mutation in Melanoma Biopsies.},
  author={P J Lamy and Florence Castan and Nicolas Concha Lozano and C{\'e}cile Mont{\'e}lion and Patricia Audran and Fr{\'e}d{\'e}ric Bibeau and Sylvie Roques and Fr{\'e}d{\'e}ric Montels and Anne-Claire Laberenne},
  journal={The Journal of molecular diagnostics : JMD},
  year={2015},
  volume={17 4},
  pages={366-73}
}
The detection of the BRAF V600E mutation in melanoma samples is used to select patients who should respond to BRAF inhibitors. Different techniques are routinely used to determine BRAF status in clinical samples. However, low tumor cellularity and tumor heterogeneity can affect the sensitivity of somatic mutation detection. Digital PCR (dPCR) is a next-generation genotyping method that clonally amplifies nucleic acids and allows the detection and quantification of rare mutations. Our aim was to… CONTINUE READING
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